Effect of acute exercise on patella tendon protein synthesis and gene expression
نویسندگان
چکیده
Evidence suggests that habitual loading can result in patellar tendon hypertrophy, especially at the proximal and distal parts of the patellar tendon. The underlying protein kinetic changes and its regulation remains controversial and human data, investigating this topic, are limited. The present study investigated how acute exercise affects growth factor production and collagen fractional synthetic rate in patellar tendon tissue from patients undergoing an anterior cruciate ligament reconstruction operation. The operation was performed by use of the bone-patellar tendon-bone method under spinal anesthesia. Twelve subjects were randomized to one of two groups: a control group or an exercise group (1-hr unilateral knee-extension 67% of Wmax 24 hours before operation). Two hours before the anterior cruciate ligament operation a flooding-dose of L-[1-(13)C]proline was given. Tissue from the most proximal part of the patellar tendon was obtained during the operation. Tendon collagen fractional synthetic rate and mRNA concentrations of TGF-β-1, CTGF, and type I and III collagen were measured. CTGF and type I collagen expression were higher in the exercise group compared to the control group (p < 0.05). Type III collagen expression (p = 0.11), TGF-β-1 expression (p = 0.34), and collagen fractional synthetic rate (p = 0.26) did not differ between groups. Although the expression of CTGF and type I collagen were higher, the patellar tendon collagen fractional synthetic rate was not correspondingly higher after exercise. The elevated CTGF expression in the exercise group indicates that the TGF-beta pathway could be an important link between mechanical loading and stimulation of tendon tissue type I collagen expression.
منابع مشابه
Comparison of the Alterations of Gene Expression Related to Signaling Pathways of Synthesis and Degradation of Skeletal Muscle Protein Induced by Two Exercise Training Protocols
Background and Objectives: Skeletal muscle mass depends on the balance between synthesis and degradation of muscle protein, which changes with aging and disease. The aim of the present reserch was to examine the effects of two exercise training protocols on alterations of some genes involved in pathways of protein synthesis and degradation in order to achieve a more effective training program i...
متن کاملComparing the effects of endurance and resistance trainings on gene expression involved in protein synthesis and degradation signaling pathways of Wistar rat soleus muscle
Background: Skeletal muscle mass, which is regulated by a balance between muscle protein synthesis and degradation, is an important factor for movement to meet everyday needs, especially in pathological conditions and aging. The purpose of the present investigation was to compare the alterations of the gene expression involved in muscle protein synthesis and degradation signaling pathways induc...
متن کاملThe Effect of 8-Weeks of Low-Intensity Swimming Training on Promyelocytic Leukemia Zinc Finger Protein and Spermatid Transition Nuclear Protein Gene Expression in Azoospermic Rats Model
Aims: One of the causes of infertility in men is the azoospermia disease, which is attributed to the lack of sperm in each sperm. The primary function of spermatogenesis is the maintenance, proliferation, and differentiation of spermatogonial cells. Thus, the present study aimed to investigate the changes in Promyelocytic Leukemia Zinc Finger (PLZF) and spermatid Transition Nuclear Protein (TNP...
متن کاملEffects of eight weeks aerobic training on kynurenine and gene and protein expression of aryl hydrocarbon receptor in the heart of male rats
Introduction: Understanding the molecular cellular mechanisms of the heart is essential for the prevention and treatment of cardiovascular disease. This study aimed was to investigate the effect of aerobic exercise on the kynurenine and gene and protein expression of aryl hydrocarbon receptor (AHR) in the heart tissue of healthy male Wistar rats. Materials and Methods: The present study is an e...
متن کاملEffect of Cardiac Rehabilitation Program Based on Combined Training on VEGF/Endostatin Gene Expression Ratio in Patients with Acute Coronary Syndrome
Background: Coronary artery disease is one of the most common causes of death in the world. With the increase in the incidence of these diseases, surgical and non-surgical interventions followed by cardiovascular rehabilitation programs have become more important. The process of angiogenesis and improvement of blood flow is considered as one of the therapeutic goals in these patients, and vascu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 2 شماره
صفحات -
تاریخ انتشار 2013